dc.contributor.author	Frank, Julia
dc.contributor.author	Richter, Maximilian
dc.contributor.author	de Rossi, Chiara
dc.contributor.author	Lehr, Claus-Michael
dc.contributor.author	Fuhrmann, Kathrin
dc.contributor.author	Fuhrmann, Gregor
dc.contributor.department	HIPS, Helmholtz-Institut für pharmazeutische Forschung Saarland, Universitätscampus 8.1, 66123 Saarbrücken, Germany.	en_US
dc.date.accessioned	2018-09-17T07:54:12Z
dc.date.available	2018-09-17T07:54:12Z
dc.date.issued	2018-08-17
dc.description.abstract	Extracellular vesicles (EVs) are natural nanoparticles that play important roles in intercellular communication and are increasingly studied for biosignalling, pathogenesis and therapy. Nevertheless, little is known about optimal conditions for their transfer and storage, and the potential impact on preserving EV-loaded cargoes. We present the first comprehensive stability assessment of different widely available types of EVs during various storage conditions including -80 °C, 4 °C, room temperature, and freeze-drying (lyophilisation). Lyophilisation of EVs would allow easy handling at room temperature and thus significantly enhance their expanded investigation. A model enzyme, β-glucuronidase, was loaded into different types of EVs derived from mesenchymal stem cells, endothelial cells and cancer cells. Using asymmetric flow field-flow fractionation we proved that the model enzyme is indeed stably encapsulated into EVs. When assessing enzyme activity as indicator for EV stability, and in comparison to liposomes, we show that EVs are intrinsically stable during lyophilisation, an effect further enhanced by cryoprotectants. Our findings provide new insight for exploring lyophilisation as a novel storage modality and we create an important basis for standardised and advanced EV applications in biomedical research.	en_US
dc.identifier.doi	10.1038/s41598-018-30786-y
dc.identifier.issn	2045-2322
dc.identifier.pmid	30120298
dc.identifier.uri	http://hdl.handle.net/10033/621482
dc.rights	Attribution-NonCommercial-ShareAlike 3.0 United States	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-sa/3.0/us/	*
dc.source.journaltitle	Scientific reports
dc.title	Extracellular vesicles protect glucuronidase model enzymes during freeze-drying.	en_US
dc.type	Article	en_US
dspace.entity.type	Publication
refterms.dateFOA	2018-09-17T07:54:13Z

Extracellular vesicles protect glucuronidase model enzymes during freeze-drying.

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