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CHEMICALLY MODIFIED ELECTRODES AS BIOSENSORS: AMPEROMETRIC GLUCOSE SENSOR FOR FLOW INJECTION ANALYSIS
Yacynych, Alexander M. Sasso, Sylvia V. Reynolds, Eugene R. Geise, Robert J.
Yacynych, Alexander M.
Sasso, Sylvia V.
Reynolds, Eugene R.
Geise, Robert J.
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Issue Date
1987
Submitted date
2023-07-19
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Abstract
An amperometric biosensor was constructed for glucose blood serum
determinations with flow injection analysis. A platinized, reticulated
vitreous carbon (RVC) electrode is used as an enzyme support, reactor,
and detector. A partial platinum coating provided an increased current
response from the oxidation of hydrogen peroxide, as compared with a
bare RVC electrode. It also allowed the electrode to operate at a much
lower potential (+0.6 vs +0.9 volts vs SCE), thus resulting in an
increased signal to noise ratio. However, carbonaceous sites were still
available for enzyme immobilization. Glucsseloxidase (E.eC2 1T.1IE 3.4) is
immobilized on the surface of the RVC electrode to provide selectivity
for glucose and to produce an electroactive product, hydrogen peroxide.
The enzyme is immobilized by either covalent attachment with
carbodiimide or cross-linking with glutaraldehyde. The enzyme remained
active for over three months with almost daily use. An
electropolymerized film of 1,2-diaminobenzene was incorporated on the
sensor to eliminate the effects of electrochemically active
interferences present in serum samples. The polymer film also virtually
eliminated electrode fouling caused by large proteins. The working
lifetime of the sensor, as well as, the thermal stability of the
immobilized glucose oxidase were increased by the polymer film.
Citation
Biosensors International Workshop 1987, 69 - 74
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF Monographs, Volume 10
ISSN
0930-4320
EISSN
ISBN
0-89573-683-7
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Attribution-NonCommercial-ShareAlike 4.0 International