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Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells.
Schubert, Maren Nimtz, Manfred Bertoglio, Federico Schmelz, Stefan Lukat, Peer van den Heuvel, Joop
Schubert, Maren
Nimtz, Manfred
Bertoglio, Federico
Schmelz, Stefan
Lukat, Peer
van den Heuvel, Joop
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2021-05-21
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Abstract
he expression of mammalian recombinant proteins in insect cell lines using transient-plasmid-based gene expression enables the production of high-quality protein samples. Here, the procedure for virus-free transient gene expression (TGE) in High Five insect cells is described in detail. The parameters that determine the efficiency and reproducibility of the method are presented in a robust protocol for easy implementation and set-up of the method. The applicability of the TGE method in High Five cells for proteomic, structural, and functional analysis of the expressed proteins is shown.
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Methods Mol Biol. 2021;2305:129-140. doi: 10.1007/978-1-0716-1406-8_6.
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Article
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en
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1940-6029
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Attribution 4.0 International