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RECORD OF THE TECHNICAL DISCUSSION AT THE SYMPOSIUM
Reichenbach, Hans
Reichenbach, Hans
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1975
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2023-01-31
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Abstract
The majority of the people present voted for having also in future a
symposium on myxobacteria every year. To allow sufficient time for
planning and raising funds, however, the place of the meeting should
be decided upon 2 years in advance. The meeting should alternate regularly
between both sidesof the Atlantic.
An invitation has been extended by our colleagues in California to
arrange the next meeting at the Asilomar Conference Grounds, Pacific
Grove, California. The invitation was gratefully accepted, and July
25 to 27 was suggested as the most desirable time for the meeting.
(This date has been verified in the meantime. H.R.)
Ian Sutherland proposed that he would contact Colin Clarke and Howard
Parish for chances to arrange the 1977 meeting in the United Kingdom.
This was approved.
The majority decided to restrict also future symposia on the biology
of myxobacteria and to exclude other gliding bacteria. However, people
from other fields of research may be invited to participate in the
meeting.
There was agreement not to publish a detailed symposium's report. Only
the summaries of the lectures should be distributed.
In order to be able to trace back quickly experimental strains to their
origin, it was agreed upon to suggest for general adaption the labelling
system which was proposed at the Cold Spring Harbor meeting: Each
strain should be identified by the initials of the individual who isolated
the strain or, if this is not feasable, who first introduced it
into the literature. Furthermore, different strains of one species
coming from one person should be numbered consecutively, the numbering
beginning anew with another species; e.g. Myxococcus xanthus RBl,
strains is independent of designations given the strain in individual
laboratories, and should always remain attached to the name of the
strain whenever anything is published about the strain and regardless
who works with it later on. When a strain is sent to another individual,
care should be taken to supply the original labelling with the strain.
The complete history of the strain should be given when the strain is
introduced into the literature for the first time. (Note added when
writing down the Record: to avoid repeated labelling of one strain
care should be taken to adopt as label the initials of the person who
originally isolated and distributed the organism, for he might have
sent the same strain to different laboratories. If necessary the label
should be inquired from the original source of the strain. H.R.)
Robert P. Burchard has been asked at the Cold Spring Harbor meeting
to work out a system for the designation of mutants. His proposals
have already been made public in the Myxobacterial Messenger. They
were unanimously approved, suggested for general use, and may be cited
here again:
The system is based on that proposed by Demerec et al. (Genetics 54:
61 - 76, 1966).
"Briefly, genus and species names should be followed by the initials
of the describing investigator (first and last names). He or she would
then assign a number to the strain, starting with 1. Each investigator
should keep a log book with a list of his strains, their origin
and history, and their genotype and/or phenotype. The latter could be
accomplished with Demerec et al "3 letter plus" abbreviations. Using
my strains as an example:
M. xanthus RB1 derived from Dworkin's FB as a stable tan and called
strain FB, to date. The phenotype nomenclature would be Tan-1.
RB4 is my non-motile NM, derived from SM which in turn is derived
from FB.
The phenotype designation Cam-r25 means resistant to 25 ug chloramphenicol/
ml.
For genotype, lower case lettering is used. Thus, a methionine-requiring
auxotroph might be designated as "met-1". The hyphen could
be replaced by a letter (capital ) when mapping is accomplished".
It was again urged to make better use of the Myxobacterial Messenger
(M.M.) to our mutual benefit. Completed Ph.D. theses on gliding bacteria
should be reported to the M.M. and a brief abstract supplied.
Planned gatherings of myxobacteria people at ASM meetings should be
announced early in the M.M..
As already suggested at the Cold Spring Harbor meeting each laboratory
should sent a list of available strains, including mutants, to
the M.M..
At the Cold Spring Harbor meeting it has been suggested to start
a central collection of all myxobacterial strains including mutants
available. Martin Dworkin inquired in the meantime from Ellis Brockman
if he would be willing and in a position to keep such a collection.
This is not the case. Hans Reichenbach promised to ask at the Deutsche
Sammlung von Mikroorganismen (German Collection of Microorganisms) in
Göttingen whether they would be willing to accept such a collection.
(Note added when writing down the Record: The policy of the Deutsche
Sammlung von Mikroorganismen does not allow to keep such a specialized
collection. H.R.).
Citation
2nd International Symposium on the Biology of Myxobacteria, 16
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