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FIBER OPTICAL pH-SENSOR BASED ON LASER-INDUCED FLUORESCENCE

Czolk, R.
Gantner, E.
Ache, H. J.
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Issue Date
1987
Submitted date
2023-09-06
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Abstract
A fiber optical pH-sensor based on laser-induced fluorescence was developed. The fluorescence was ww. with the light of a Helium-Neon-Laser (5°mW, Polytec model PL 7504) emitting at X4 632.8 nm. The dye used was tetra-(p-sulfophenyl)-porphyrine (Ventron), ‘whieh-has in an aqueous solution an extinction coefficient at A = 632.8 nm of e = 4365 I/mol - cm. Its fluorescence behaviour changes at different-pH-values- (figure). It is a water-soluble dye and the sulfo-groups give the possibility to immobilize this dye on anion exchange resins. The fluorescence spectrum of this dye has a maximum at pH = 6.8 at A= 675 nm and A= 700 nm, respectively, which offers the advantage of a ratio-measurement.
Citation
Biosensors International Workshop 1987, 307 - 308
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Type
Book chapter
conference paper
Language
en
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Series/Report no.
GBF Monographs, Volume 10
ISSN
0930-4320
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ISBN
0-89573-683-7
3-527-26801-4
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Attribution-NonCommercial-ShareAlike 4.0 International