Loading...
RhoG and Cdc42 can contribute to Rac-dependent lamellipodia formation through WAVE regulatory complex-binding.
Schaks, Matthias Döring, Hermann Kage, Frieda Steffen, Anika Klünemann, Thomas Blankenfeldt, Wulf Stradal, Theresia Rottner, Klemens
Schaks, Matthias
Döring, Hermann
Kage, Frieda
Steffen, Anika
Klünemann, Thomas
Blankenfeldt, Wulf
Stradal, Theresia
Rottner, Klemens
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
2019-08-26
Submitted date
Files
Loading...
Open Access article
Adobe PDF, 3.15 MB
Loading...
supplementary figure S1
TIFF, 8.27 MB
Loading...
supplementary figure S2
TIFF, 10.03 MB
Other Titles
Abstract
Cell migration frequently involves the formation of lamellipodial protrusions, the initiation of which requires Rac GTPases signalling to heteropentameric WAVE regulatory complex (WRC). While Rac-related RhoG and Cdc42 can potently stimulate lamellipodium formation, so far presumed to occur by upstream signalling to Rac activation, we show here that the latter can be bypassed by RhoG and Cdc42 given that WRC has been artificially activated. This evidence arises from generation of B16-F1 cells simultaneously lacking both Rac GTPases and WRC, followed by reconstitution of lamellipodia formation with specific Rho-GTPase and differentially active WRC variant combinations. We conclude that formation of canonical lamellipodia requires WRC activation through Rac, but can possibly be tuned, in addition, by WRC interactions with RhoG and Cdc42.
Citation
Small GTPases. 2019 Aug 26:1-11. doi: 10.1080/21541248.2019.1657755.
Publisher
Journal
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
2154-1256
EISSN
ISBN
ISMN
Gov't Doc #
Sponsors
License
Attribution-NonCommercial-ShareAlike 4.0 International