Loading...
Increased neutrophil extracellular trap-mediated Staphylococcus aureus clearance through inhibition of nuclease activity by clindamycin and immunoglobulin.
Schilcher, Katrin Andreoni, Federica Uchiyama, Satoshi Ogawa, Taiji Schuepbach, Reto A Zinkernagel, Annelies S
Schilcher, Katrin
Andreoni, Federica
Uchiyama, Satoshi
Ogawa, Taiji
Schuepbach, Reto A
Zinkernagel, Annelies S
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
2014-08-01
Submitted date
Other Titles
Abstract
The Gram-positive human pathogen Staphylococcus aureus causes a variety of human diseases such as skin infections, pneumonia, and endocarditis. The micrococcal nuclease Nuc1 is one of the major S. aureus virulence factors and allows the bacterium to avoid neutrophil extracellular trap (NET)-mediated killing. We found that addition of the protein synthesis inhibitor clindamycin to S. aureus LAC cultures decreased nuc1 transcription and subsequently blunted nuclease activity in a molecular beacon-based fluorescence assay. We also observed reduced NET degradation through Nuc1 inhibition translating into increased NET-mediated clearance. Similarly, pooled human immunoglobulin specifically inhibited nuclease activity in a concentration-dependent manner. Inhibition of nuclease activity by clindamycin and immunoglobulin enhanced S. aureus clearance and should be considered in the treatment of S. aureus infections.
Citation
Increased neutrophil extracellular trap-mediated Staphylococcus aureus clearance through inhibition of nuclease activity by clindamycin and immunoglobulin. 2014, 210 (3):473-82 J. Infect. Dis.
Publisher
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
1537-6613