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Potentiometric Immunoassay (PIA)- Mixed Potential Shift by an Antigen/Antibody Reaction
Meyer, Heinrich Cammann, Karl
Meyer, Heinrich
Cammann, Karl
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Issue Date
1992
Submitted date
2024-04-10
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Abstract
This paper presents a method for the direct potentiometric detection of a specific antigen/antibody
reaction. The method is based on a principle called "modulation of a mixed potential" at an ionselective
electrode (ISE). The ion-selective membrane of an ISE is covered with antibody resp.
antigen molecules. A high sensitivity is obtained when the mixed potential is especially unstable
which is in general the case if a mixed potential between cations andanionsis built up across the
phase boundary ofthe ion-selective membrane towards the sample solution. If the antigen binds to
its specific antibody, a directly detectable shift results in the mixed potential. If the
"immunoelectrode" is completed by a reference electrode, the corresponding changes of the cell
voltage vs. concentration of antigen resp. antibody follows a typical immunological calibration
curve. Since no labeled antigen Tesp. antibody molecule is needed, the necessary ISE is simple to
construct andthe instrumentation is quite teasonable, PIA will be a very attractive method.
Citation
Biosensors : fundamentals, technologies and applications, 83 - 86
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
EISSN
ISBN
3527284370
1560812206
1560812206
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Attribution-NonCommercial-ShareAlike 4.0 International