Loading...
A Novel Mechanism of Host-Pathogen Interaction through sRNA in Bacterial Outer Membrane Vesicles.
Koeppen, Katja Hampton, Thomas H Jarek, Michael Scharfe, Maren Gerber, Scott A Mielcarz, Daniel W Demers, Elora G Dolben, Emily L Hammond, John H Hogan, Deborah A
Koeppen, Katja
Hampton, Thomas H
Jarek, Michael
Scharfe, Maren
Gerber, Scott A
Mielcarz, Daniel W
Demers, Elora G
Dolben, Emily L
Hammond, John H
Hogan, Deborah A
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
2016-06
Submitted date
Files
Loading...
Open Access publication
Adobe PDF, 2 MB
Other Titles
Abstract
Bacterial outer membrane vesicle (OMV)-mediated delivery of proteins to host cells is an important mechanism of host-pathogen communication. Emerging evidence suggests that OMVs contain differentially packaged short RNAs (sRNAs) with the potential to target host mRNA function and/or stability. In this study, we used RNA-Seq to characterize differentially packaged sRNAs in Pseudomonas aeruginosa OMVs, and to show transfer of OMV sRNAs to human airway cells. We selected one sRNA for further study based on its stable secondary structure and predicted mRNA targets. Our candidate sRNA (sRNA52320), a fragment of a P. aeruginosa methionine tRNA, was abundant in OMVs and reduced LPS-induced as well as OMV-induced IL-8 secretion by cultured primary human airway epithelial cells. We also showed that sRNA52320 attenuated OMV-induced KC cytokine secretion and neutrophil infiltration in mouse lung. Collectively, these findings are consistent with the hypothesis that sRNA52320 in OMVs is a novel mechanism of host-pathogen interaction whereby P. aeruginosa reduces the host immune response.
Citation
A Novel Mechanism of Host-Pathogen Interaction through sRNA in Bacterial Outer Membrane Vesicles. 2016, 12 (6):e1005672 PLoS Pathog.
Publisher
Journal
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
1553-7374