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DEVELOPMENT OF ELECTROCHEMICAL FIA SYSTEM WITH IMMOBILIZED ENZYMES AS SPECIFIC POST-COLUMN DETECTOR OF HPLC
Yao, Toshio
Yao, Toshio
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Issue Date
1991
Submitted date
2024-02-07
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Abstract
A sensitive and highly selective method for the simultaneous determination
of a variety of species is proposed. An amperometric flowinjection
system with the immobilized enzyme reactors or enzyme sensors
is used as the specific post detection system of HPLC, to convert
compounds separated by a reversed-phase HPLC column to electroactive
species (hydrogen peroxide and uric acid) which can be detected at 0.5
V vs. Ag/AgCl. The proposed system has been applied to the optically
specific detection of L- and D-amino acids, the simultaneous detection
of acetylcholine and choline, specific detection of a group of purine
bases and purine nucleosides, and the high-sensitive detection of Llactate
and pyruvate with substrate amplification. Specificity of the
method is proposed by the HPLC column, the enzymatic reactions, and the
electrochemical detector.
Citation
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 217 - 224
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 14
ISSN
0930-4320
EISSN
ISBN
1560811218
3527282491
3527282491
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Attribution-NonCommercial-ShareAlike 4.0 International